TABLE 1.
Primers and probes used in this study
| Primer or probe | Sequence from 5′ to 3′a | Position | GenBank no. | Amplicon length (bp) |
|---|---|---|---|---|
| ail-F | TTTGGAAGCGGGTTGAATTG | 17797–17778 | FR729477.2 | 101 |
| ail-R | GCTCACGGAAAGGTTAAGTCATCT | 17697–17720 | ||
| ail probe | FAM-CTGCCCCGTATGCCATTGACGTCTTA-BHQ | 17747–17772 | ||
| foxA-F | ACGGCGGTGATGTGAACAA | 386606–386624 | AM286415.1 | 85 |
| foxA-R | GGGTCCACTTGCAGCACATT | 386690–386671 | ||
| foxA probe | FAM-ACCTTCCTTGATGGGCTGCGCTTACTC-BHQ | 386626–386652 | ||
| IAC-F | GCAGCCACTGGTAACAGGAT | 1216–1235 | L09137 | 118 |
| IAC-R | GCAGAGCGCAGATACCAAAT | 1314–1333 | ||
| IAC probe | HEX-AGAGCGAGGTATGTAGGCGG-TAMRA | 1240–1259 |
a
BHQ, black hole quencher; FAM, 6-carboxyfluorescein; HEX, 5-hexachloro-fluorescein; TAMRA, 6-carboxytetramethylrhodamine.