Table 4.

Electroporation of DRG.

Parameter	Martinez	Ketschek	Chadborn	Dib-Hajj	Kirton	Lonza	MacGillavry	McCall	Pristera	YU
Year	2003	2010	2006	2009	2013	–	2009	2012	2012	This work
Species	Chick	Chick	Rat	Rat or Mouse	Rat	Mouse	Rat	Rat	Rat	Mouse
Sex M: Male; F: Female	–	–	–	n.c.	M	–	M	n.c.	n.c.	M/F
Age	embryo E9-E12	embryo E7	embryo E18	neonatal or adult	7 day old	embryonic or adult	adult	adult	adult	embryo E14.5
Cell number	2.5–12.5 × 106	2 × 106 c	1 × 106	2 × 106	2 × 106	5 × 104 or 2 × 104	0.2–1 × 106 c	3 × 105	5 × 104	6–9 × 104
DNA (μg)	0.5–1.25	10d	5	2+10a; 1+5b	2	0.1–0.6	n.c.e	2	≥1	1.5–2.5
Electroporation volume (μl)	25	100c	100	100	100	20	20c	20	10	10
Apparatus	BTX Electro Square Porator T820 Genetronics	Nucleofector® II Lonza	Nucleofector®	Nucleofector®	Nucleofector® 2B	Nucleofector® II - “S”	Nucleofector® 96-well system	Nucleofector®-4D X-unit	Neon® Life Technologies	Neon®
Program	Phosphate Buffered Saline 1mM CaCl2 5.5 mM glucose	G-013	G-13	G-013 rat DRG O-003 mouse DRG	O-03	SNC Basic Neuro Program 6 Nucleofector Solution	c	DR114/P3 Buffer	Buffer R	Buffer R
Voltage V/cm	1120	–	–	–	–	–	–	–	1200	1300
Pulse duration (ms)	5	–	–	–	–	–	–	–	20	20
Number of Pulses	1	–	–	–	–	–	–	–	2	2
Efficiency (48 h)	12%	n.c.	n.c.	5–20%	n.c.	≈60%f	siRNA ≥50%	40%	n.c.	67%
Survival	n.c.	n.c.	n.c	n.c.	n.c.	≈40%f	n.c.	18–27%	n.c.	>60%

Summary of existing methodology reports describing electroporation/nucleofection of DRG from embryonic Chick, Rat (embryonic and adult) and Mouse (adult), compared to the study presented herein. Unless otherwise stipulated, efficiency and survival are at 48 h post-electroporation; n.c., not communicated.

^aadult Rat or mouse (10 = Carrier DNA);

^bneonate Rat (5 = Carrier DNA);

^cauthors communicated electroporation was performed according to manufacturer's protocol;

^dmanufacturer suggests 1–2 μg DNA;

^eexpression plasmids and siRNA were used. No indication of amount of DNA;

^f24 h post-electroporation.