Figure 1.

Azot Is Expressed during Cell Selection of Viable Unfit Cells

(A–M) Expression analysis of Azot during different types of cell competition. For all pictures, Azot::dsRed reporter (A) is in red, and merges show outcompeted clones (green, marked with GFP) of several genotypes. DAPI is in blue. The following genotypes were analyzed: (B and C) azot:dsRed and (D–F) tub>dmyc background (black) and WT cells marked with GFP (green). Clones were generated as shown in (D) and analyzed 48 hr ACI. (G and H) tub>dmyc background (black) and WT cells marked with GFP (green) expressing in addition to the P35 caspase inhibitor (UASp35). Forty-eight hourr ACI. (I–M) Flip-out clones (green) generated as shown in (I) and overexpressing brinker (UASbrinker) (J and K), fwe^Lose-B (UASfwe^Lose-B) (L and M), or mfwe³(UASmfwe³).

(Q and R) Twenty-four hour ACI.

(N–P, S, and T) General overexpression of UASfwe^Lose-B and UASmfwe³ using the actin promoter as shown in (N).

(U–Y) Pupal retinas at different developmental time points. (U and V) Expression analysis of Azot (red), using Azot::dsRed, in peripheral photoreceptors at 40 hr after pupa formation (APF) (U and V). (W) Genomic engineering strategy used for the generation of azot knockout (KO) flies. (X and Y) GFP expression (green) driven by the azot promoter in azot{KO; gfp}, 44 hr APF, DAPI (blue, Y).