Figure 2.
Azot Is Required to Eliminate Loser Cells and Unwanted Neurons
(A–F) Analysis of azot KO during dmyc-induced supercompetition 72 hr ACI. (D) Quantification of cleaved caspase-3 and GFP-positive cells during dmyc-induced supercompetition in azot+/+ and azot−/− backgrounds (p < 0.01) 72 hr ACI. (E) Quantification of number of clones; the following backgrounds were analyzed: (A and E) azot+/+, (E) azot+/− (p < 0.01), (B and E) azot−/− (p < 0.01), and (C and E) azot−/−;+/+ (p > 0.05). (F) Percentage of the wing pouch occupied by the wt cells in the (A and F) azot+/+, (F) azot+/−, (B and F) azot−/−, (C and F) azot−/−;+/+.
(G–L) Role of azot during neuronal culling in the pupal retina. (K and L) Quantification of the number of apoptotic (TUNEL-positive, magenta) or Hid-expressing (red) peripheral photoreceptors, in azot+/+ (G, H, K, and L) and azot−/− (p < 0.01) (I, J, K, and L) flies. DAPI is in blue.
(M–Q) Hid expression (red) in loser clones (green) during supercompetition 48 hr ACI in azot+/+ (M, N, and Q) and azot−/− (O–Q) backgrounds.
(R–T) Seventy-two hour ACI mfwe3-overexpressing clones (UASmfwe3) in azot+/+ (R and T) and azot+/− (S and T) backgrounds (p < 0.01).
(U–W) Analysis of an extra genomic copy of azot during dmyc-induced supercompetition. (U) Quantification of the number of clones during dmyc-induced supercompetition with or without an extra genomic copy of azot. (V and W) Discs analyzed 48 hr ACI in azot+/+ (V) and azot+/+; azot+ (p < 0.01) (W).
(X) Azot expression is required for cell-competition-mediated apoptosis of loser cells. Data are represented as mean ± SEM.