Figure 3.
Azot Mutants Show Developmental Aberrations
(A–E) Wings of 10- to 13-day-old flies and quantification of developmental aberrations in the wing of each genotype, ∗∗ < 0.01. (A and B) azot+/+, (A and C) azot−/−;azot+/+, (A and D) azot−/− and (A and E) azot+/+;azot+.
(F–K) Azot and cleaved caspase-3 expression upon UV irradiation (2 × 10−2 J irradiation dose during second instar larvae, treatment as shown in F). (G) Quantification of the percentage of Azot and cleaved caspase-3-expressing cells after UV irradiation. (H) Azot::dsRed expression after UV irradiation (red), (I) cleaved caspase-3 (green) after UV irradiation, (J) merge, and (K) merge with DAPI (blue).
(L–P) Quantification of developmental aberrations and images of wings from 10- to 13-day-old flies after UV treatment (2 × 10−2 J, pupae stage 0) of genotypes (L and M) azot+/+, (L and N) azot−/−;azot+/+, (L and O) azot−/−, and (L and P) azot+/+;azot+.
(Q) Scheme showing the requirement of azot function for preventing developmental aberrations. Data are represented as mean ± SEM.