Figure S1.

Azot Is Conserved throughout Evolution and Is Expressed in a Subset of Loser Cells in Cell Competition Scenarios, Related to Figure 1

(A) Alignment of Azot showing conservation in multicellular animals including humans. Point mutations highlighted for the generation of the pm4Q12 Azot mutant.

(B) Expression profile of different genes induced upon Supercompetition based on microarray data published in Rhiner et al., 2010.

(C) In situ analysis of azot RNA in dmyc-induced supercompetition, azot RNA probe (red), WT clones (green). Arrows show cells expressing azot RNA.

(D and E) HA-tagged Azot protein overexpressed in wing imaginal disc cells with act-Gal4 driver is mainly cytoplasmic, anti-HA in red (D) and merged with DAPI (E).

(F–U) Expression analysis of Azot. Flip-out overexpressing clones of UASdaxin (Azot::dsRed, red) (F) and RNAihopscotch (Azot::dsRed, red) (G). (H) Minute heterozygous clones anti-Azot antibody (red). (I and J) Wing imaginal discs ubiquitously expressing Daxin and GFP (act < Gal4; UASdaxin; UASgfp), (I) Azot::dsRed and merged with GFP (J). (K–O) Flip-out clones marked with GFP and overexpressing: (K) UASlacZ, (L) UAShid, (M) UASeiger, (N) UASbax, and (O) RNAiscribble. Azot expression revealed with Azot::dsRed from (K)–(O). (P and Q) patched-Gal4; UASgfp; UASCSK-IR, (red, Azot::dsRed). (R–U) Images of pupal retinas at different developmental time points. Expression analysis of Azot (red), using Azot::dsRed, in peripheral photoreceptors at different time points: 36hr after pupa formation (APF) (R and S) and 38hr APF (T and U).