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Efficacy of fgf10b knockdown. A: Diagram showing the exon/intron structure of the fgf10b locus. Above the diagram are primer-binding sites for PCR, and below are target sites for translation-blocking MO (tbmo) and splice-blocking MOs (e1i1mo) and (e2i2mo). B: RT-PCR amplification of fgf10b mRNA at 12 hpf in control embryos or embryos injected with the indicated mounts of splice-blocking MO. Expression of ornithine decarboxylase (odc) at 12 hpf serves as a control.
