FIG. 6.

Histological analysis of the newly formed muscle tissues. After myogenic in vivo differentiation, tissues formed by unlabeled (control) and 400 μg/mL labeled hMPCs were harvested and evaluated by (a) H&E staining and immunostaining for muscle markers desmin, myosin heavy chain (MyHC), and α-actinin 4 weeks after MPC injection; scale bar is 50 μm and (b) Prussian blue staining for iron after 1, 2, and 4 weeks after injection, with a higher magnification shown in (c) Scale bar is 50 μm. (d) Uptake of SPIO nanoparticles by macrophages was evaluated 4 weeks after injection via co-staining with Prussian blue and marker F4/80 for mouse macrophages. Scale bar is 100 μm. H&E, hematoxylin and eosin. Color images available online at www.liebertpub.com/tec