Number of Nanog- and Gata4-positive cells for different doses of small molecules. (A) SB431542, (B) ACTIVIN A, (C) Forskolin, (D) leukemia inhibitor factor (LIF), and (E) dimethyl sulfoxide (DMSO). P<0.05 is considered significant. ***P<0.001, **P<0.01, and *P<0.05, when compared with control.