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. Author manuscript; available in PMC: 2015 Feb 2.
Published in final edited form as: Cell Calcium. 2009 Aug 18;46(3):219–225. doi: 10.1016/j.ceca.2009.07.004

Fig. 1.

Fig. 1

Serca2 gene and flox targeting vector design. Map of the relevant region of the Serca2 (Atp2a2) gene with non-coding exons (open boxes) and coding exons (filled boxes). Exon numbers are indicated. Non-genomic DNA is indicated by thick lines. * indicates the 5′ and 3′ ends of the cloned Serca2 genomic fragment. Restriction sites: B, BamH1; H, HindIII; K, KpnI; N, NotI; X, XhoI; Xc, XcmI; S, SalI; Sp, SpeI. Genomic probes for detection of 5′ end, exon1 and 3′ end recombination events are indicated. Triangles denote loxP recombination sites. Neo and TK, Neo and HSV-TK cassettes; wt, wild-type Serca2 gene; pSerca2T, the targeting vector; Serca2T, the allele generated by homologous recombination between the Atp2a2 gene and pSerca2T targeting vector; Serca2flox, the allele generated from Serca2T by partial Cre excision; Serca2Δ, the null allele generated from Serca2flox in cells in which Cre recombinase is expressed.