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Impact of 4 weeks vs. 8 weeks of CUS on thymocyte subsets. Percentage of main cell populations in thymus after exposure to 4 (A) and 8 (B) weeks of CUS. Flow cytometry plot showing the gating strategy for thymocyte subsets (gate) (C). DN, double-negative thymocytes; DP, double-positive thymocytes; and SPCD4 and SPCD8, single-positive CD4 and CD8 thymocytes, respectively. Each bar represents the mean ± SEM from 10 animals per group. *p < 0.05.
