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RFLP conditions used for digestion.
| Size (size) | Position | Restriction enzyme | Product size (bp) | |
|---|---|---|---|---|
| 775 | L263E | ApoI | L: 775 | E: 633 + 142 |
| E431K | MBoII | E: 299 + 241 + 117 + 69 + 49 | K: 416 + 241 + 69 + 49 | |
| 141 | A623E | Cac8I | A: 114 + 27 | E: 141 |
| 164 | S769N | DdeI | S: 136 + 28 | N: 164 |
