Table 4.

Interlaboratory Control Data for Female Pubertal Assay

	Lab #1			Lab #2			Overall values
Endpoint	Mean	SD	CV	Mean	SD	CV	Mean	SD	CV
BW initial (g)	51.3	3.20	6.2	49.6	1.29	2.6	50.8	2.84	5.6
Final BW (g)	158.2	8.06	5.1	154.8	3.72	2.4	157.1	7.11	4.5
Age at VO (days)	35.4	0.99	2.8	34.6	1.02	2.9	35.2	1.05	3.0
Age at incomplete VO (days)	34.7	0.91	2.6	NA	NA	NA	34.7	0.91	2.6
Body wt at VO (g)	123.6	6.63	5.4	113.4	5.05	4.5	120.5	7.76	6.4
Age at first estrus (days)	36.8	0.95	2.6	35.6	1.00	2.8	36.4	1.11	3.0
Estrous cycle length (days)	4.7	0.27	5.7	4.7	0.18	3.8	4.7	0.24	5.1
Percent cycling	98.7	2.76	2.8	94.4	7.11	7.5	97.4	4.80	4.9
Percent regularly cycling	81.3	13.69	16.8	84.4	9.29	11.0	82.2	12.39	15.1
Liver wt (g)	7.4	0.63	8.5	7.2	0.21	2.9	7.3	0.54	7.4
Kidney wt (g)	1.3	0.08	6.2	1.3	0.04	3.1	1.3	0.07	5.4
Pituitary wt (mg)	9.7	1.03	10.6	7.9	0.20	2.5	9.1	1.20	13.2
Adrenal wt (mg)	34.8	1.78	5.1	35.5	3.85	10.8	35.0	2.52	7.2
Ovarian wt (mg)	74.6	4.87	6.5	57.5	3.80	6.6	69.4	9.21	13.3
Uterine wet wt (mg)	302.6	33.04	10.9	315.5	12.70	4.0	306.5	28.72	9.4
Uterine blotted wt (mg)	256.9	22.19	8.6	280.4	17.66	6.3	264.0	23.30	8.8
Thyroid wt (mg)	9.3	1.32	14.2	8.8	0.33	3.8	9.2	1.13	12.3
Blood urea nitrogen (BUN) (mg/dl)	13.2	1.25	9.5	12.7	1.11	8.7	13.1	1.21	9.2
Creatinine (mg/dl)	0.1	0.05	50.0	0.1	0.02	20.0	0.12	0.04	33.3
Serum T4 levels (μg/ml)	4.4	0.27	6.1	3.8	0.25	6.6	4.2	0.37	8.8
Serum TSH levels (ng/ml)	6.4	1.60	25.0	3.6	0.82	22.8	5.5	1.92	34.9

Lab #1: n = 16; Lab #2: n = 7.

BW, body weight; VO, vaginal opening; NA, not applicable; wt, weight.