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. 2015 Feb 2;8:468. doi: 10.3389/fncel.2014.00468

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Copyright © 2015 Boassa, Nguyen, Hu, Ellisman and Sosinsky.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Colocalization of Panx2 with cellular markers indicates early endosomal sorting. In (A), untagged WT Panx2 (red) and four organellar markers (green) were co-immunolabeled with the polyclonal anti-C terminal Panx2 antibody and a monoclonal antibody specific to each organellar proteins. The four protein markers, Clathrin, Adaptin β, Rab4 and p47A label clathrin-coated vesicles, early/recycling endosomes, early endosomes, and degradation vesicles derived from the Golgi, respectively. Both the single plane confocal images (A) and the Percent Colocalization (Manders' Coefficient × 100) graph in (B) indicated that Panx2 overlaps with early endosomes. Sample sizes for Manders' Coefficient calculation: 107 (clathrin), 119 (rab4), 131 (adaptin β) and 144 (p47A) cells. The errors bars indicate standard deviations.