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Characterization of chondrogenic OPTIMAIX constructs (post 3 weeks of perfusion culture). (A) Representative histological Safranin-O staining for GAG and immunohistochemical staining for type-II collagen indicating cartilaginous tissue formation (n = 4). Scale bar = 1 mm (valid for both panels). (B) Gene expression levels for MSC cultured on scaffolds for 3 weeks. Measurements are mean ± SD (n = 3).
