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. 2015 Feb 2;3:10. doi: 10.3389/fbioe.2015.00010

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Copyright © 2015 Hoffmann, Feliciano, Martin, de Wild and Wendt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of loaded and non-loaded constructs following the hypertrophic culture phase. The panels depict histological Safranin-O staining, immunohistochemical staining for collagen type-II, alizarin red (AR) staining and immunohistochemical staining for collagen type-X, and bone sialoprotein (BSP), respectively (n = 4). Loaded constructs show a higher degree of maturation as compared to non-loaded constructs. Scale bar = 1 mm (valid for all panels).