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. Author manuscript; available in PMC: 2015 Feb 2.
Published in final edited form as: Biochemistry (Mosc). 2011 Nov;76(11):1262–1269. doi: 10.1134/S0006297911110083

Fig. 3.

Fig. 3

Wild-type cortactin enhances GLUT4myc translocation in L6 and CHO cells. a) Cells were transiently transfected with cortactin GFP cDNA, cortactin-GFP localizes to both cytoplasmic perinuclear compartments (white arrows) and submembranous compartments (pink arrows). b) Cells expressing wild-type cortactin-GFP are indicated by white arrows. GLUT4myc translocation is significantly greater in L6-GLUT4myc cells overexpressing wild type cortactin (white arrows) than in control cells that do not express cortactin-GFP (pink arrow) in the presence of 300 nM insulin (upper panels). Actin staining (lower panels) shows that insulin stimulates the formation of actin stress fibers equally in L6-GLUT4myc cells overexpressing wild type (WT) cortactin-GFP (white arrows) as well as in control cells (pink arrows). c) Similar results were obtained using CHO-GLUT4myc cells. d) Cytochalasin D completely inhibits GLUT4 translocation (upper panels) and formation of actin stress fiber (lower panels) in response to insulin in L6-GLUT4myc cells with (white arrows) and without (pink arrows) the overexpression of wild type cortactin. Green fluorescence indicates GFP, red fluorescence indicates GLUT4myc or phalloidin staining.