Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1976 Nov;73(11):4184–4186. doi: 10.1073/pnas.73.11.4184

Role of antigenic structure in cell to cell cooperation.

M Schwartz, R J Hooghe, E Mozes, M Sela
PMCID: PMC431377  PMID: 1087024

Abstract

Two synthetic polypeptides which differ only in the order of amino acids in their NH2-terminal side chains, namely, (Tyr-Tyr-Glu-Glu)-poly(DLAla)- -poly(LLys) and (Tyr-Glu-Try-Glu)-poly(DLAla)- -poly(LLys), were found to be under different genetic control. By three different in vivo systems for thymus-derived cell depletion, it was demonstrated that (Tyr-Tyr-Glu-Glu)-poly(DLAla)- -poly(LLys), which represents the random poly(Tyr,Glu)-poly(DLAla)- -poly(Lys) in the pattern of immune responses and in the quality of antibodies they elicit, is thymus-dependent whereas (Tyr-Glu-Tyr-Glu)-poly(DLAla)-poly(LLys) does not require thymus-derived cell help for efficient antibody production. Therefore, the two ordered polypeptides which are similar chemically differ in parameters, not yet determined, which affect their capability to trigger bone marrow-derived cells.

Full text

PDF
4186

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson B., Blomgren H. Evidence for thymus-independent humoral antibody production in mice against polyvinylpyrrolidone and E. coli lipopolysaccharide. Cell Immunol. 1971 Oct;2(5):411–424. doi: 10.1016/0008-8749(71)90052-9. [DOI] [PubMed] [Google Scholar]
  2. Armstrong W. D., Diener E., Shellam G. R. Antigen-reactive cells in normal, immunized, and tolerant mice. J Exp Med. 1969 Feb 1;129(2):393–410. doi: 10.1084/jem.129.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bonavida B., Mozes E., Shearer G. M., Sela M. Immunological unresponsiveness induced in adult mice to synthetic polypeptides built on multichain polyproline and multichain polyalanine. Immunochemistry. 1974 Jul;11(7):347–353. doi: 10.1016/0019-2791(74)90187-6. [DOI] [PubMed] [Google Scholar]
  4. Claman H. N., Chaperon E. A., Triplett R. F. Immunocompetence of transferred thymus-marrow cell combinations. J Immunol. 1966 Dec;97(6):828–832. [PubMed] [Google Scholar]
  5. Feldmann M. Cell interactions in the immune response in vitro. II. The requirement for macrophages in lymphoid cell collaboration. J Exp Med. 1972 May 1;135(5):1049–1058. doi: 10.1084/jem.135.5.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Feldmann M., Easten A. The relationship between antigenic structure and the requirement for thymus-derived cells in the immune response. J Exp Med. 1971 Jul 1;134(1):103–119. doi: 10.1084/jem.134.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Feldmann M., Howard J. G., Desaymard C. Role of antigen structure in the discrimination between tolerance and immunity by b cells. Transplant Rev. 1975;23:78–97. doi: 10.1111/j.1600-065x.1975.tb00150.x. [DOI] [PubMed] [Google Scholar]
  8. Feldmann M. Induction of immunity and tolerance in vitro by hapten protein conjugates. I. The relationship between the degree of hapten conjugation and the immunogenicity of dinitrophenylated polymerized flagellin. J Exp Med. 1972 Apr 1;135(4):735–753. doi: 10.1084/jem.135.4.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gorczynski R. M., Feldmann M. B Cell heterogeneity - difference in the size of B lymphocytes responding to T dependent and T independent antigens. Cell Immunol. 1975 Jul;18(1):88–97. doi: 10.1016/0008-8749(75)90039-8. [DOI] [PubMed] [Google Scholar]
  10. Hardy B., Mozes E., Danon D. Comparison of the immune response potential of newborn mice to T-dependent and T-independent synthetic polypeptides. Immunology. 1976 Feb;30(2):261–266. [PMC free article] [PubMed] [Google Scholar]
  11. Howard J. G., Christie G. H., Courtenay B. M., Leuchars E., Davies A. J. Studies on immunological paralysis. VI. Thymic-independence of tolerance and immunity to type 3 pneumococcal polysaccharide. Cell Immunol. 1971 Dec;2(6):614–626. doi: 10.1016/0008-8749(71)90009-8. [DOI] [PubMed] [Google Scholar]
  12. Jaton J. C., Sela M. Role of optical configuration in the immunogenicity and specificity of synthetic antigens derived from multichain polyproline. J Biol Chem. 1968 Nov 10;243(21):5616–5626. [PubMed] [Google Scholar]
  13. Lichtenberg L., Mozes E., Shearer G. M., Sela M. The role of thymus cells in the immune response to poly(Tyr, Glu)-polyD L Ala--polyLys as a function of the genetic constitution of the mouse strain. Eur J Immunol. 1974 Jun;4(6):430–434. doi: 10.1002/eji.1830040609. [DOI] [PubMed] [Google Scholar]
  14. McDevitt H. O., Sela M. Genetic control of the antibody response. I. Demonstration of determinant-specific differences in response to synthetic polypeptide antigens in two strains of inbred mice. J Exp Med. 1965 Sep 1;122(3):517–531. doi: 10.1084/jem.122.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McDevitt H. O., Tyan M. L. Genetic control of the antibody response in inbred mice. Transfer of response by spleen cells and linkage to the major histocompatibility (H-2) locus. J Exp Med. 1968 Jul 1;128(1):1–11. [PMC free article] [PubMed] [Google Scholar]
  16. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mozes E., Schwartz M., Sela M. Antibody response of inbred mouse strains to ordered tetrapeptides of tyrosine and glutamic acid attached to multichain polyalanine or polyproline. Tyr-Tyr-Glu-Glu is a major determinant of the random poly-(Tyr, Glu)-polyDLAla--polyLys. J Exp Med. 1974 Aug 1;140(2):349–355. doi: 10.1084/jem.140.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mozes E., Shearer G. M. Contribution of bone marrow cells and lack of expression of thymocytes in genetic controls of immune responses for two immunopotent regions within poly-(Phe,Glu)-poly-Pro--poly-Lys in inbred mouse strains. J Exp Med. 1971 Jul 1;134(1):141–161. doi: 10.1084/jem.134.1.141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Möller G., Michael G. Frequency of antigen-sensitive cells to thymus-independent antigens. Cell Immunol. 1971 Aug;2(4):309–316. doi: 10.1016/0008-8749(71)90065-7. [DOI] [PubMed] [Google Scholar]
  20. Möller G. One non-specific signal triggers b lymphocytes. Transplant Rev. 1975;23:126–137. [PubMed] [Google Scholar]
  21. Raff M. C. Role of thymus-derived lymphocytes in the secondary humoral immune response in mice. Nature. 1970 Jun 27;226(5252):1257–1258. doi: 10.1038/2261257a0. [DOI] [PubMed] [Google Scholar]
  22. Sela M., Mozes E., Shearer G. M. Thymus-independence of slowly metabolized immunogens. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2696–2700. doi: 10.1073/pnas.69.9.2696. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sela M., Mozes E. The role of antigenic structure in B lymphocyte activation. Transplant Rev. 1975;23:189–201. doi: 10.1111/j.1600-065x.1975.tb00158.x. [DOI] [PubMed] [Google Scholar]
  24. Shearer G. M., Mozes E., Sela M. Contribution of different cell types to the genetic control of immune responses as a function of the chemical nature of the polymeric side chains (poly-L-prolyl and poly-DL-alanyl) of synthetic immunogens. J Exp Med. 1972 May 1;135(5):1009–1027. doi: 10.1084/jem.135.5.1009. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES