. Author manuscript; available in PMC: 2015 Feb 2.

Published in final edited form as: Nat Chem Biol. 2007 Jul 15;3(8):480–485. doi: 10.1038/nchembio.2007.9

Table 1.

LC/MS data acquired for early PTT biosynthetic intermediates

Sample	Compound	Formula	Retention time (min)^a	Ion detected	Calculated (m/z)	Measured (m/z)^b
WM6492 (ΔphpC)	AEP	C₂H₈NO₃P	22.5/ 22.5	[M−H]⁻	124.0169	124.0168/ 124.0169
PhpC in vitro	HEP	C₂H₇O₄P	15.8/ 16.4	[M−H]⁻	125.0009	125.0010/ 125.0009
WM6601 (ΔphpD)	HEP	C₂H₇O₄P	16.2/ 16.4	[M−H]⁻	125.0009	125.0009/ 125.0009
PhpD in vitro	HMP	CH₅O₄P	15.7/ 16.9	[M−H]⁻	110.9853	110.9852/ 110.9853
WM6602 (ΔphpE)	HMP	CH₅O₄P	17.6/ 16.9	[M−H]⁻	110.9853	110.9852/ 110.9853
WM6488 (ΔphpJ)	AMPn	CH₆NO₃P	22.1/ 22.8	[M−H]⁻	110.0013	110.0011/ 110.0013
PhpF in vitro	CMP-5’-PF	C₁₀H₁₅N₃O₁₂P₂	9.6	[M+H]⁺	432.0204	432.0203
PhpF in vitro	CMP-5’-PF, NH₄⁺	C₁₀H₁₅N₃O₁₂P₂	9.6	[M+NH₄]⁺	449.0469	449.0468

^a,b

Retention times and measured mass to charge ratio (m/z) values are listed such that the first value represents our experimental results and the second value is derived from the analysis of a corresponding authentic standard (if available).