Fig. 3.
pALTINK4a/b expression is induced by oncogenic stimuli. (A) Naked mole rat cells were subjected to anchorage-independent growth to trigger anoikis by placing them in plates precoated with 1% agarose for 48 h. RNA was extracted from the cells, and expression from INK4a/b locus was analyzed by qPCR. (B) Naked mole rat cells were transfected with plasmids encoding HRas V12 or BRAF E600 oncogenes or pControl plasmid encoding GFP. RNA was extracted 10 d after transfection, and expression from INK4a/b locus was analyzed by qPCR. Four lines of low-passage naked mole rat skin fibroblasts were used for each experiment. Error bars indicate SD; *P < 0.05; **P < 0.005; Student’s t test.