Fig. 5.
pALTINK4a/b overexpression attenuates γ-irradiation–induced apoptosis in NSF2 mut cells. NSF2 mut cells were transfected with plasmids encoding HPRT (control), p15INK4b, p16INK4a, or pALTINK4a/b under the CMV promoter. Two days after transfection, cells were subjected to 20 Gy γ-irradiation. (A) At four and 10 d after γ-irradiation, the cells were harvested, and the percent of apoptotic cells was analyzed by Annexin-V staining and flow cytometry. (B) Cells were photographed 10 d after γ-irradiation. Control plate shows many floating apoptotic cells whereas plates transfected with INK4a/b products show lower numbers of apoptotic bodies and many growth-arrested cells. The experiments were repeated three times, and error bars show SD; statistical significance is indicated by asterisk. *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.00005, Student’s t test.