Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 12;112(4):1179–1184. doi: 10.1073/pnas.1415466112

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

PMC Copyright notice

Fig. 2. — Native PfRH5, PfRipr, and CyRPA interact to form a 200-kDa multiprotein complex in the parasite. (A) Schizont lysate analyzed by anion-exchange chromatography detected PfRH5, PfRipr, and CyRPA to coelute at 350 mM NaCl (peak 1). (B) SEC-200 analysis of peak 1 proteins detected PfRH5, PfRipr, and CyRPA in fractions (32–36) corresponding to 200 kDa. BN-PAGE of SEC fraction 34 (C) and culture supernatant (D) confirmed that PfRH5, PfRipr, and CyRPA coexisted as an intact 200-kDa native protein complex. *PI represents the corresponding preimmune sera. (E) SEC-200 analysis of culture supernatant also detected PfRH5, PfRipr, and CyRPA in fractions (35–38) corresponding to ∼200 kDa. β-Amylase and alcohol dehydrogenase (ADH) are SEC molecular weight standards.