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. 2015 Jan 12;112(4):1179–1184. doi: 10.1073/pnas.1415466112

Fig. 4.

Fig. 4.

Invasion inhibitory efficacy of the CyRPA and PfRipr-1/-2 antibodies. (A) Total rat IgGs containing antibodies against the corresponding recombinant proteins were evaluated for their invasion inhibitory activity in one-cycle (40 h after invasion) and two-cycle (90 h after invasion) assays against the P. falciparum strain 3D7. Full-length CyRPA-FL IgGs exhibited potent dose dependent invasion inhibition. (B) Anti-CyRPA antibodies exhibited strain-transcending invasion inhibition. (C) CyRPA antibodies in presence of a fixed concentration of PfRH5 (1 mg/mL) antibodies exhibited synergistic inhibition in one cycle (40 h after invasion) against the sialic acid independent strain, 3D7, (D) as well as a sialic acid dependent strain, MCamp. Blue curve represents the invasion inhibitory activity of the individual CyRPA-FL antibodies. Green curve represents the invasion inhibition observed with varying concentration of CyRPA-FL antibodies in presence of a fixed concentration of anti-PfRH5 IgG (1 mg/mL). Red curve represents the theoretical invasion inhibitory activity predicted by Bliss additivity. Anti-PfRH5 IgGs used at 1 mg/mL gave an invasion inhibition of 26.27(±2.17) and 19.4 (±2.4) for 3D7 and MCamp, respectively. The error bars represent the SEM of three independent experiments performed in duplicate. *P ≤ 0.05; **P ≤ 0.01.