Fig. 2.

Quantal release characteristics at PII–GC synapses. (A) Representative peak amplitude distribution of a PII–GC pair recorded in 2 (gray) and 0.5 mM (cyan) extracellular Ca²⁺. (Left) Curves represent MP-CBA functions. (Right) Representative uIPSCs including failures. (B) MP-CBA–based estimates of p_rs, q (Left), and N_rs (Right) for six pairs with different uIPSC peak amplitudes. Note that q tends to increase with uIPSC peak amplitude. (C) First-latency distribution (black) corrected according to Barrett and Stevens (BS) (26) (gray). Curve represents γ-function fit (8) to BS-corrected data. (Inset) Amplitude-normalized and superimposed uIPSC (black), quantal IPSC (gray), and reconvolved uIPSC (cyan). (D) t₅₀ of the BS- or Minneci et al. (MS) (27)-corrected first-latency distributions do not correlate with τ of uIPSCs.