TABLE 1 .
d37‐41gp42 titin linker insertion mutantsa
| Mutant gp42 | Length of insert (no of amino acids) | Sequence of linker insert |
|---|---|---|
| d37‐41(I27‐1) | 92 | ALIEVEKPLYGVLVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDAEIIEDGKKHILILHNAQLGMTGEVSFQAANAKSAANLKVKELPR |
| d37-41 (I27-1,2) | 184 | ALIEVEKPLYGVLVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDAEIIEDGKKHILILHNAQLGMTGEVSFQAANAKSAANLKVKELPRRSLIEVEKPLYGVLVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDAEIIEDGKKHILILHNAQLGMTGEVSFQAANAKSAANLKVKELPR |
| d31-44 (I27-1,2,3,4) | 368 | ALIEVEKPLYGVLVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDAEIIEDGKKHILILHNAQLGMTGEVSFQAANAKSAANLKVKELPRALIEVEKPLYGVLVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDAEIIEDGKKHILILHNAQLGMTGEVSFQAANAKSAANLKVKELPRALIEVEKPLYGVLVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDAEIIEDGKKHILILHNAQLGMTGEVSFQAANAKSAANLKVKELPRALIEVEKPLYGVLVFVGETAHFEIELSEPDVHGQWKLKGQPLTASPDAEIIEDGKKHILILHNAQLGMTGEVSFQAANAKSAANLKVKELPR |
a Titin immunoglobulin-like 127 domains were PCR amplified from clones kindly provided by Andreas Matouschek (21). Following PCR, bands representing one, two, three, and four titin domainss were digested with PasI and ligated into a unique PasI in d37-41gp42.