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Growth of E. coli O78-9 and its iss mutants in 50% serum. For this experiment, we used E. coli strains O78-9, its mutant deleted for the iss gene (Δiss), and its derivative cured of the ColV plasmid (ΔpColV). Cultures were grown overnight in minimal MOPS medium and diluted into the same medium with or without 50% serum. Growth was monitored at an optical density or absorbance of 600 nm, and growth curves were generated on a Biotek Eon platform.
