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. 2015 Feb 2;10(2):e0116251. doi: 10.1371/journal.pone.0116251

Figure 3. Methiones oxidation impairs CaM interaction with FADD and TRADD.

Figure 3

A: CaM oxidation analysis. Coomassie blue stained 15% SDS-PAGE loaded with the indicated Xenopus laevis His-CaM proteins. B. Schematic representation of GST-FADD and GST-TRADD proteins used in pull-down assays. The DED, DD and the S194 phosphorylation site of human FADD are indicated. C: GST pull-down assays. The indicated GST proteins bound to gluathione-sepharose beads were incubated with recombinant His-CaM proteins, as indicated; 50% of the eluted proteins were subjected to SDS-PAGE (12%) and western blot analysis. I indicates the input of the CaM proteins used in binding assays. The data shown are representative of at least three independent experiments.