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. 2015 Feb 2;10(2):e0116251. doi: 10.1371/journal.pone.0116251

Figure 4. Effect of Met-to-Leu substitutions in CaM on FADD and TRADD binding.

Figure 4

A: CaM mutants and SDS-PAGE analysis. Coomassie stained gel (a) and western blot analysis (b) of CaM mutants, identified by a numerical code detailed in Table 1. B: GST pull-down assays. Native (N) or oxidized (Ox) CaM mutants were incubated with the indicated GST proteins bound to glutathione-sepharose beads. 50% of the eluted proteins were analyzed by 12% SDS-PAGE and blotted to nitrocellulose stained with ponceau (a) and processed for western blot (b). I indicates the input of recombinant CaM proteins (250 ng). CaM mutants are identified by a numerical code as in Table 1. A and B: the black vertical lines in all panels indicate that non-adjacent lanes from the same gel or blots are shown. The data shown are representative of three independent experiments.