Table 1. Met-to-Leu CaM mutants.
| CaM mutants | CaM domains: Met and Leu positions | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Code | Description | N-terminal | Linker | C-terminal | ||||||
| 36 | 51 | 71 | 72 | 76 | 109 | 124 | 144 | 145 | ||
| 1 | wild type | M | M | M | M | M | M | M | M | M |
| 2 | 9L | L | L | L | L | L | L | L | L | L |
| 3 | 8L 109M | L | L | L | L | L | M | L | L | L |
| 4 | 8L 124M | L | L | L | L | L | L | M | L | L |
| 5 | M36–76L | L | L | L | L | L | M | M | M | M |
| 6 | M124–145L | M | M | M | M | M | M | L | L | L |
| 7 | M51–72L | M | L | L | L | M | M | M | M | M |
| 8 | M36/76–145L | L | M | M | M | L | L | L | L | L |
Methionine residues located in the N-teminal (Met 36, 51, 71, 72), C-terminal (Met 109, 124, 144, 145) and linker region (Met 76) of CaM are listed. Mutant description reflects the relative position of Met-to-Leu substitutions as schematically indicated; 9L all nine methionine mutated, 8L 109M and 8L 124M eight methionine mutated with the exception respectively of residues 109M or 124M.