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The effects of chitosan on miR-29b, TGF-β1, p-Smad3 and P21 expression in fibroblasts. Fibroblasts were treated with chitosan (0 μg/ml, 5 μg/ml, 10 μg/ml and 20 μg/ml) for 24 hours. The expression of miR-29b was detected by real-time PCR (A), TGF-β1, p-Smad3 and P21 were measured by western blotting (B). *P < 0.05 by Student’s t-test, indicates a significant difference from the group without chitosan treatment. All experiments were repeated three times.
