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Primer sequences and reaction conditions
| Primer pair (5’-3’, forward and reverse) | Tm (°C) | Cycles | Size (bp) |
|---|---|---|---|
| CaBP-9k | |||
| F: ATG AGC GCT AAG AAA TCT | 55 | 30 | 287 |
| R: TCA TTC TGATAA CTT TTT GA | |||
| GAPDH | |||
| F: AAC GGA TTT GGC CGT ATT | 54 | 25 | 300 |
| R: AGC CTT CTC CAT GGT GAA GA |
