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miR-29a induced colonic epithelial HT29 cell apoptosis by down-regulating Mcl-1. A. miR-29a down-regulated mcl-1 in colonic epithelial HT29 cells but the expression of apoptosis protein Caspase-3 was increased based on Western blot analysis. GAPDH was loaded as internal control. B. The apoptosis rate of HT29 cells was significantly higher with the transfection of miR-19a plasmid. Data are expressed as the means ± SD (n = 3). *P < 0.05 vs. HT29 cells supplemented with serum (-).
