Analysis of effector molecules in Hh. A. The relative mRNA levels for SHH, SMO, and PTCH, which were detected by RT-PCR in DU-145 cells. B, C. The relative protein levels of SHH, SMO, and PTCH, which were detected by Western blotting in control, control siRNA, and siRNA LKB1. Representative quantitation from three independent experiments. The values of DU-145 are expressed relative to the respective controls (β-actin), which were given an arbitrary value of 1. Bars, SE. *P < 0.05.