Table 1.

Prime sequence, product size, cycles and Renaturation temperature of different gene

Gene	Prime sequence(5’-3’)	Product size	Cycles	Renaturation temperature (°C)
SHH	F: GACTCAGAGGTGTAAGGACAAGTT	85 bp	30	59
	R: CTCGGTCACCCGCAGTTT
SMO	F: CAACCTCTTTGCGTTTCCTT	154 bp	30	59
	R: ACTCACTGCTCCTATCCCACTC
PTCH	F: CAGGCAGCGGTAGTAGTGG	193 bp	25	59
	R: TGTAGCGGGTATTGTCGTGT
LKB1	F: CGGCAAGGTGAAGGAA	141 bp	25	59
	R :ACGCCCAGGTCGGAGAT
GAPDH	F: GGGAAACTGTGGCGTGAT	299 bp	25	56
	R: GAGTGGGTGTCGCTGTTGA