Figure 3.

PLGF promote migration in siha cells through activating Flt-1 signaling pathway. A. Western blot analysis of Flt-1 expression in siha cells infected with a shRNA lentivirus against Flt-1. Flt-1 expression was significantly decreased in siha cells expressing Flt-1-shRNA. The bar graph shows the relative protein expression levels among groups. β-actin was used as a loading control. Data are presented as mean ± SD. for three independent experiments. *P < 0.05. B. PLGF promoted the migration of siha cells in vitro through Flt-1 signaling pathway. PLGF induced a 2-fold increase in migration of siha cells compared with the controls. Reduction of Flt-1 expression inhibited the PLGF-mediated migration of siha cells. *P < 0.05.