Figure 4.

PLGF regulates EMT-related proteins expression in siha cells. Western blot analysis of mesenchymal cell markers and epithelial cell markers in siha cells when Flt-1 was activated by PlGF or down-regulated by shRNA. Western blot analysis of mesenchymal cell markers and epithelial cell markers in siha cells when Flt-1 was activated by PlGF or down-regulated by shRNA. β-actin was used as a loading control for whole cell lysate, The bar graphs show the relative expression of proteins among each treatment groups. Data are presented as mean ± SD. for three independent experiments. *P < 0.05.