Figure 2. HCWEs inhibit HSV binding and penetration to host cells.

(A) HCWEs inhibited virus binding to host cells. 1X10⁶ Vero cells were cooled to 4°C and exposed to 100 pfu HSV-1 (filled square), HSV-2 (filled circle), and HSV-AR (filled triangle) with indicated concentrations of HCWEs. Plaque assays were used to determine the inhibitory effect of HCWEs on the virus binding stage. (B) HCWEs inhibited virus penetration into host cells. 1X10⁶ Vero cells were infected with 100 pfu HSV-1 (filled square), HSV-2 (filled circle), and HSV-AR (filled triangle) for 2 hours at 4°C and then incubated with HCWEs at 37°C to allow viral penetration. Plaque assays were used to determine the inhibitory effect of HCWEs on the virus penetration stage.