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. 2015 Feb 2;10(2):e0116839. doi: 10.1371/journal.pone.0116839

Figure 1. Formation of 3D-MBs under hESC conditions.

Figure 1

(A) Survey of melanoma cell lines for the ability to form attached 3D-spheroids in hESC medium. The 1205Lu cell line formed attached 3D-spheroids, the 451Lu cell line formed floating spheroids, while the other tested cell lines displayed only various degrees of aggregation. (B) Formation of attached 1205Lu 3D-MBs after transfer to hESC medium over time. (C) To confirm that 1205Lu MBs formed by aggregation, 1205Lu GFP-labeled cells and 1205Lu nuclear dsRed-labeled were mixed and cultured as described in Panel A. (D) Mature 1205Lu 3D-MBs were removed by pipetting and were re-plated under standard monolayer media growth conditions, under which they reseeded a new monolayer. Shown is monolayer outgrowth after 96 hours.