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. 2015 Feb 2;10(2):e0116839. doi: 10.1371/journal.pone.0116839

Figure 4. Neural stem cell features of 3D-MBs.

Figure 4

(A) Data extracted from 1205Lu microarray analysis shows upregulation of neural stem cell genes in MB versus OM samples, normalized to average expression of three housekeeping genes, GAPDH, ACTB and HSP90. Error bars indicate standard deviation. (B) Analysis of neural stem cell gene upregulation in 1205Lu MBs using quantitative reverse transcription real time PCR, as normalized to GAPDH expression. Errors bars represent the standard deviation from triplicate samples. (C) Western blot analysis after switching 1205Lu monolayer cells to hESC conditions. (D) Immuofluorescence detection of neural stem cell markers in 1205Lu MBs and monolayer cultures. (E) Dual staining of MBs with TUJ1 and SOX2 antibodies. (F) Heterotypic MBs formed with 1205Lu and GFP-labeled HeLa cells were transferred to standard media, resulting in HeLa cells with neural features migrating from MBs.