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. 2015 Feb 2;10(2):e0116839. doi: 10.1371/journal.pone.0116839

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© 2015 Peretz et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Diagram of experimental design. (B) Representative images showing differentiation phenotype of 1205Lu cells after ID4 siRNA knockdown, hESC media challenge (five days), and switching to 254 medium (96 hours). (C) Western blot testing of ID4 knockdown using shRNAs delivered by lentivectors. (D) Representative images showing differentiation phenotype of 1205Lu cells after ID4 shRNA knockdown and hESC media challenge. (E) Representative images showing differentiation phenotype after ID4 shRNA delivery to 1205Lu cultures without exposure to hESC medium. Phenotype was enhanced after 96 hours in 254 medium.