Fig. 5.

The costimulation and/or restimulation of TLR7 and TLR9 decrease the expression of TLR7 and TLR9. (A) RAW264.7 cells were stimulated with either an agonist for TLR7 or TLR9 or both overnight (1st), subsequently the cells were washed with PBS and incubated with culture media overnight. After incubation, the cells were restimulated with TLR agonists for 1 h (2nd) and the expression of TLR7 and TLR9 was measured using RT-PCR. (B) The cells were incubated with either an agonist for TLR7 and TLR9 or both for 4 or 16 h, and the expression of endosomal TLR7 and TLR9 was measured using flow cytometry. The data are presented as the mean fluorescence intensity (MFI) (C).