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. 2014 Nov 26;38(1):33–39. doi: 10.14348/molcells.2015.2163

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Fig. 1. — Reagents used for Redβ expres sion and ssOR assays. (A) Structure of Redβ expression plasmid. (B) Structure of plasmid carrying the neo* target for gene correction. (C) Sequence surrounding the 4 bp insertion (bold underlined) in neo*. (D) Sequence of control (CO) and repair (RO) ssOs used in ssOR assays. (E) Immunoblot analysis of Redβ expression in hygro^R clones isolated after cotransfection of HT1080 cells with the plasmids shown in (A) and (B). Clones were expanded and grown continually with tetracycline (Tet) in the growth medium (+), or for 3 days after the removal of tetracycline (−), before analysis.