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The TLR2/4 pathway is required for activation of JNK/p38. RAW 264.7 cells were transfected with siTLR4 (A) or siTLR2 (B) for 24 h and then infected with S. pneumoniae for 0, 1, 2, or 4 h. Subsequently, the cell lysates were analyzed by Western blotting. The band densities were measured by ImageJ. The data are representative of three independent experiments and were analyzed by one-way ANOVA. Data show mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001.
