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Co-immunoprecipitation (IP) of A2A and CB1 receptors in crude (P2 fraction) and Percoll-purified striatal synaptosomes. CB1 receptors were readily detected and enriched in complexes immunoprecipitated with the anti-A2A receptor antibody, but not with mouse IgG2A, either in P2 or Percoll-purified fractions of rat striatal synaptosomes. The label ‘input (3%)’ refers to the protein load corresponding to 3% of the protein quantity used for the IP.
