Figure 2.

VSC2 suppresses expression of proinflammatory enzymes in activated microglia. BV-2 cells were exposed to various concentrations of VSC2 and 0.2 μg·mL⁻¹ LPS. (A, C) The cells were harvested after 6 h and RT-PCR (gel photos above) and real-time RT-PCR (histograms below) were performed against iNOS and COX-2, using GAPDH as an internal control. (B,D) The cells were harvested after 24 h and Western blot analyses were performed against iNOS and COX-2, using β-actin as an internal control. The data are expressed as % of LPS-treated ± SEM; *P < 0.05, **P < 0.01 versus LPS-treated. (E) Viability of BV-2 cells that had been exposed to various concentrations of VSC2 for 24 h was assessed by intracellular ATP assay. No changes in viability were observed.