Figure 4.

VSC2 suppresses NF-κB signalling in activated microglia. (A) BV-2 cells were treated with various concentrations of VSC2 for 1 h and co-treated with 0.2 μg·mL⁻¹ LPS for an additional hour. NF-κB in the nuclear fraction was estimated by Western blot analysis. The same blot was subjected to Western blot against lamin B as an internal control and the cytosolic marker protein hsp90 as a negative control. After densitometry, the data were normalized against lamin B. Data are expressed as % of LPS-treated control ± SEM. (B) BV-2 cells were transfected with a plasmid containing NF-kB-luciferase reporter construct. After 48 h, the cells were treated with various concentrations of VSC2 and 0.2 μg·mL⁻¹ LPS for 6 h. Luciferase activity in the cell lysate was measured. Data are expressed as induction fold of untreated control ± SEM; **P < 0.01 versus LPS-treated.