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. 2014 Dec 23;172(4):1114–1123. doi: 10.1111/bph.12985

Figure 1.

Figure 1

Effect of 9-phenanthrol on the smooth muscle membrane potential of mesenteric arteries and block with TRAM-34. Panel A, (i–ii) intracellular records showing reproducible and reversible hyperpolarization to 20 μM 9-phenanthol (9-PA) in the same preparation (20 min washout), respective resting membrane potentials (RMPs) were −61.5 and −58.8 mV. (iii) In a separate, single experiment, the hyperpolarization to 9-PA was unaffected by the presence of 100 nM apamin, RMP −53.2 mV, (iv) but blocked in the additional presence of 1 μM TRAM-34, RMP −50.1 mV. Under these conditions, hyperpolarization was subsequently evoked with 5 μM levcromakalim (LVK) to −81.3 mV. Panel B–D, box and whisker plots summarizing (B) the effect of 20 μM 9-phenanthrol against RMP (left panel, n = 10) and in the presence of 5 μM glibenclamide (Glib, n = 5), (C) lack of block with 100 nM apamin alone and block with 1 μM TRAM-34 alone (n = 4 in each case), (D) the effect of [Na+]o replacement with NMDG on the RMP (n = 10). *, P < 0.05 vs. RMP; #, P < 0.05 vs. Control 9-PA.