Figure 3. LegC7 does not delay non-endosomal vacuolar traffic.

(A) Wild type yeast strains expressing GFP-Vam3 [58] and expressing either LEGC7 ⁺ or LEGC7 ^N242I were grown in selective media supplemented with 2% glucose at 30°C, washed in ddH₂O, suspended in fresh CSM-uracil-lysine/2% galactose, incubated at 30°C for 16 h, then visualized. (B) Wild type or atg19∆ cells expressing either LEGC7 ⁺ or LEGC7 ^N242I were grown in selective media containing 2% glucose at 30°C, washed in ddH₂O, suspended in fresh CSM-uracil/2% galactose, incubated at 30°C for 16 h, and total proteins were extracted from equal fractions. Proteins were separated and immunoblotted for Ape1p (Rabbit 1:2000) [39] and LegC7.