Figure 4.

FMOD affects MCP-1 expression through activation of NF-κB. A) MCP-1 ELISA on ECs treated with cycloheximide (20 μg/ml) with or without FMOD (3 nM). B) MCP-1 ELISA on ECs treated with CM from pigmented (P) and nonpigmented (NP) melanocytes with or without TNF-α. C) Nonpigmented (left) and pigmented (right) melanocytes were stained with pNF-κB (green), and nuclei were counterstained with DAPI (blue). D) Western blot of pNF-κB in ECs treated with FMOD (3 nM) for 30 min. β-Actin was used as a loading control. All experiments were repeated at least 3 times. **P < 0.001.