Figure 6.

Folate deficiency alters expression of genes involved in Hcy metabolism. Total cochlear RNA of mice on normal (NF, n = 11) or FD (n = 12) diets was used to analyze expression levels of the genes of interest by real-time RT-PCR using the Rn18s gene as reference. The results are shown as the mean ± SEM of determinations made in triplicate for each animal sample. Statistical analysis by Student’s t test was carried out using GraphPad Prism, and data are considered significant when *P ≤ 0.05.